RESUMO
We evaluated four root canal sealers to determine their antimicrobial effectiveness against E. faecalis. The direct contact test was used to measure the effectiveness of the study materials and close contact between bacteria on the kinetics of bacterial growth. The agar diffusion test (ADT) was also performed for comparison. Using one-way ANOVA and the F-test, significant differences between the sealers were confirmed. Whereas BioRoot endodontic sealer had an antimicrobial effect statistically similar to the zinc oxide-eugenol control (p=.99), EndoSequence sealer and AH Plus sealer both had a significantly lower antimicrobial effect than the control (p=.0000266 and p=.0000068, respectively).
RESUMO
The objective of this study was to determine the antimicrobial potential of AH plus supplemented with bismuth lipophilic nanoparticles (BisBAL NPs) on the growth of Enterococcus faecalis isolated from patients with endodontic infections. BisBAL NPs, synthesized with the colloidal method, were characterized, in its pure form or AH Plus-absorbed, by energy-dispersive X-ray spectroscopy and scanning electron microscopy (EDS-SEM). Antimicrobial activity was evaluated with disc diffusion assays, and antibiofilm activity with fluorescence microscopy. BisBAL NP-supplemented AH Plus had a 4.9 times higher antimicrobial activity than AH Plus alone (p = 0.0001). In contrast to AH Plus alone, AH Plus supplemented with BisBAL NP inhibited E. faecalis biofilm formation. The sealing properties of AH plus were not modified by the incorporation of BisBAL NPs, which was demonstrated by a 12-day split-chamber leakage assay with daily inoculation, which was used to evaluate the possible filtration of E. faecalis. Finally, BisBAL NP-supplemented AH plus-BisBAL NPs was not cytotoxic for cultured human gingival fibroblasts. Their viability was 83.7% to 89.9% after a 24-h exposure to AH Plus containing 50 and 10 µM BisBAL NP, respectively. In conclusion, BisBAL NP-supplemented AH Plus constitutes an innovative nanomaterial to prevent re-infection in endodontic patients without cytotoxic effects.
Assuntos
Anti-Infecciosos , Nanopartículas , Materiais Restauradores do Canal Radicular , Bismuto , Enterococcus faecalis , Resinas Epóxi , HumanosRESUMO
Apexification procedures have been widely used to treat teeth with incomplete root development and pulp necrosis. The middle mesial canal (MMC) is an anatomical variation and in most cases represents a challenge during endodontic treatments. In this article, a favorable outcome is reported after apexification in a molar with MMC.
RESUMO
Una infección endodóncica persistente (IEP) representa un desafío en el tratamiento de conductos radiculares. La remoción de bacterias es complicada cuando una terapia de endodoncia convencional no es efectiva, por lo que estrategias adicionales de desinfección deben aplicarse para obtener un tratamiento exitoso. Este artículo reporta el caso de una IEP en un hombre de 58 años de edad que fue referido al posgrado de endodoncia de la Universidad Autónoma de Nuevo León. Aunque un retratamiento endodóncico fue iniciado, el paciente decidió extraerse el diente con un dentista general; un conducto recurrente y una ramificación apical fueron detectados en el diente extraído
A persistent endodontic infection (PEI) represents a challenge in root canal treatments. The bacterial removal is complicated when a conventional endodontic therapy is ineffective; therefore additional strategies of disinfection should be applied to obtain a successful treatment. This article describes a PEI case of a 58-years-old man who was referred to the Department of Endodontics of Nuevo Leon Autonomous University. Although an endodontic retreatment was initiated, the patient decided to remove his tooth with a general dentist; a recurrent canal and an apical ramification were detected in the extracted tooth
